Cryptococcus gattii R265 mRNA RNA-Seq - SRA

SRX2523180: Cryptococcus gattii R265 mRNA RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 36.4M spots, 3.6G bases, 2.5Gb downloads

Design: Wild-type Cryptococcus gattii R265 was pre-grown in YPD media (30 ºC for 24 h) and then inoculated in YNB media for a further period of growth (30 ºC for 18 h). YNB + 10 μM TPEN (for zinc deprivation) was inoculated with 1x107 cells/mL and incubated for additional 4 h at 30 ºC. Cells were collected by centrifugation (10,000 g for 10 min), and RNA was isolated by Trizol (Invitrogen) after cellular lysis via mortar and pestle. RNA integrity and concentration were assessed by electrophoresis on a 1% agarose gel and by fluorometry analysis using a Qubit fluorometer and a Quant-iT RNA assay kit according to the manufacturer’s instructions (Invitrogen). MRNA was purified from total RNA samples, processed and sequenced using Solexa technology on an Illumina Genome Analyzer GAII (Fasteris Life Sciences SA, Plan-les-Ouates, Switzerland).

Submitted by: Universidade Federal do Rio Grande do Sul

Study: Cryptococcus gattii R265 RNA-Seq

PRJNA368962 • SRP098059 • All experiments • All runs

show Abstracthide Abstract

RNA-Seq of sRNAs and mRNAs from wild-type and Zap1 mutant Cryptococcus gattii R265 in zinc deprivation culture.

Sample: Cryptococcus gattii VGII R265 WT under zinc deprivation

SAMN06276024 • SRS1944816 • All experiments • All runs

Organism: Cryptococcus deuterogattii

Library:

Name: R265_WT_mRNA

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq