SRX1774662: Macrocystis integrifolia transcriptome
2 ILLUMINA (NextSeq 500) runs: 47.3M spots, 14.3G bases, 7Gb downloads
Design: Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1g of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (MAGBIO, Cat# AC-60050). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Qubit and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). (Refer Figure 1 for Bioanalyzer profiles of amplified product ePCR1).
Submitted by: Universidad Nacional Agraria La Molina
Study:
Macrocystis integrifolia trasncriptome as related to Alginate and Fucoidan synthesis influenced by depth in the seashow Abstracthide AbstractComparative transcriptome analysis of Brown algae Macrocystis integrifolia collected from 0m and 10m depth of a peruvian sea with special focus on Alginate and Fucoidan production
Sample:
Transcriptome data of Brown algae Macrocystis integrifolia collected from a Peruvian seaLibrary:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: SINGLE
Spot descriptor:
1 forward
Runs:
2 runs, 47.3M spots, 14.3G bases, 7Gb