Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA isolation was performed using Tri-Reagent (MRC). To obtain ribosomal RNA (rRNA)-depleted total RNA, a DNase-treatment was performed with the RNase-Free DNase Set (Qiagen) and the RNeasy MiniKit (Qiagen), before using the Ribo-Zero rRNA Removal Kit (Epicentre) to remove rRNA. Library preparation was performed using the ScriptSeq v2 RNA-Seq library preparation kit (Epicentre) according to the manufacturer's protocol