show Abstracthide AbstractRecent advances in genome sequencing, genotyping and computational strategies have made it possible and affordable to develop expressed sequence tag (EST) libraries that contain hundreds of thousands to millions of sequences. ESTs, sequences of cDNA made by reverse transcription of mRNAs, are particularly valuable for discovering new genes, for obtaining data on gene expression and regulation, for constructing genomic maps and for the development of single nucleotide polymorphism (SNP) markers. The goal of this project is to develop a reference transcriptome of ESTs from multiple tissues/organelles and growth stages of Pisum sativum cv 'Aragorn' and develop an EST database.