DNA-Seq of Sorghum bicolor: pooled leaf genomic DNA - SRA

SRX21454675: DNA-Seq of Sorghum bicolor: pooled leaf genomic DNA
1 ILLUMINA (Illumina NovaSeq 6000) run: 40.5M spots, 12.1G bases, 5Gb downloads

Design: genomic DNA was randomly fragmented by sonication, then DNA fragments were end polished, A-tailed, and ligated with the full-length adapters of Illumina sequencing, and followed by further PCR amplification with indexed oligos. The PCR products as the final construction of the libraries were purified with magnetic beads. Then libraries were checked for size distribution by Bioanalyzer and quantified by real-time PCR .

Submitted by: USDA-ARS

Study: Genome sequencing for bulk segregant analysis mapping of sorghum late flowering mutants

PRJNA1006376 • SRP455665 • All experiments • All runs

show Abstracthide Abstract

Illumina sequencing of pooled genomic DNA isolated from 20 late flowering siblings from a segregating F2 mapping population.

Sample: Sorghum bicolor genomic DNA pool made from 20 late flowering F2 siblings

SAMN37113337 • SRS18690680 • All experiments • All runs

Organism: Sorghum bicolor

Library:

Name: 2x0483 pool

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 40.5M spots, 12.1G bases, 5Gb

Run	# of Spots	# of Bases	Size	Published
SRR25730657	40,467,708	12.1G	5Gb	2023-11-26

ID:: 28894772

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity