GSM6752822: Habneula Projecting VP Biol rep 4; Mus musculus; RNA-Seq - SRA

SRX18352968: GSM6752822: Habneula Projecting VP Biol rep 4; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 44.3M spots, 6.7G bases, 2.6Gb downloads

External Id: GSM6752822_r1

Submitted by: Institute for Genome Sciences

Study: Adaptations in nucleus accumbens neuron subtypes mediate negative affective behaviors in fentanyl abstinence II

PRJNA904347 • SRP409281 • All experiments • All runs

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We used RNAseq to evaluate baseline expression of NPAS+ VP translatomes in comparison to bulk VP translatomes. Overall design: We performed gene expression profiling in ribosome-associated mRNA from two different mouse genotypes (NPAS-Cre x Rpl22-HA or Rpl22-HA with Cre AAV in the VP) at baseline

Sample: Habneula Projecting VP Biol rep 4

SAMN31840643 • SRS15840477 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM6752822

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA was extracted with a DNase step (Qiagen) using the RNeasy mini kit (Qiagen #74104) by adding lysis buffer supplemented with beta mercaptoethanol.

Runs: 1 run, 44.3M spots, 6.7G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR22382765	44,304,722	6.7G	2.6Gb	2022-11-28

ID:: 25367187

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