Instrument: Illumina HiSeq 1500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Ribosome Profiling (RNAse I digestion, sucrose gradient for collecting 80S fractions, pelleting 80S, splitting 80S and footprint isolation, size selection, end repair, adapter ligation, reverse transcription, circularization and PCR amplification for seqeuncing). For ribosome profiling of Ski samples, instead of sucrose gradient, 80S were directly pelleted by sucrose cushion after RNAse I digestion.