Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. Release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. Ribosome profiling: Digest with Mnase (30 min 10 ug/ml, 37C), pellet ribosomes, extract RNA, PNK treat, gel isolation, library generation with NEB Directional Small RNA kit mRNA-seq: RiboMinus, library prepataion using TruSeq RNA Sample Preparation Kit (Illumina)