U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX1050328: GSM1704503: 6 h infection cytosol ribosome profiling (Rep 1); Homo sapiens; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 16.3M spots, 828.8M bases, 523.3Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Ribosome profiling analysis of Dengue Virus
PRJNA285961 • SRP059187 • All experiments • All runs
show Abstracthide Abstract
Dengue virus is an + strand RNA virus. We have carried our infections of human cells with Dengue and analyzed the translation, replication, and localization of the Dengue RNA. This allowed for clear definition of the life cycle of the Dengue virus inside a host cell. We also assessed the host response to Dengue virus, finding that a large fraction of the translational response is due to Interferon function. Overall design: Translational and transcriptional analysis of the cellular response to Dengue virus infection
Sample: 6 h infection cytosol ribosome profiling (Rep 1)
SAMN03763400 • SRS954647 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Pre-treatment with 180 uM cycloheximide for 30s. PBS wash. For fractionated cells, release cytosol with digitonin. Extract remaining sample with dodecyl maltoside. NEB Directional Small RNA kit PNK end polishing, linker ligation, reverse transcription, PCR amplification
Experiment attributes:
GEO Accession: GSM1704503
Links:
External link:
Runs: 1 run, 16.3M spots, 828.8M bases, 523.3Mb
Run# of Spots# of BasesSizePublished
SRR205291116,250,647828.8M523.3Mb2016-09-01

ID:
1525476

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...