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SRX15950877: GSM6283339: Wild-type_1_footprint; Saccharomyces cerevisiae; OTHER
1 ILLUMINA (Illumina HiSeq 4000) run: 19.9M spots, 1G bases, 305.8Mb downloads

External Id: GSM6283339_r1
Submitted by: MCB, UC Berkeley
Study: loss of DBP1 during meiosis in yeast
PRJNA854601 • SRP384366 • All experiments • All runs
show Abstracthide Abstract
We used CRISPR/Cas9 to delete the DBP1 ORF in SK1 budding yeast cells. We then used ribosome profiling and mRNA-seq to observe gene expression profiles of wild-type and dbp1? cells during meiosis. Overall design: Cells were grown as described in Powers et al., 2022. Samples were collected 6h post transfer to SPO media. Two biological replicates are included for each genotype.
Sample: Wild-type_1_footprint
SAMN29439890 • SRS13637522 • All experiments • All runs
Organism: Saccharomyces cerevisiae
Library:
Name: GSM6283339
Instrument: Illumina HiSeq 4000
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: cells were mixermilled with frozen buffer described in Powers et al., 2022 as described in Brar et al., 2012 except linker ligation was used instead of poly a tailing ribosome profiling and mrna-seq
Runs: 1 run, 19.9M spots, 1G bases, 305.8Mb
Run# of Spots# of BasesSizePublished
SRR1990811819,858,8031G305.8Mb2022-11-29

ID:
22619444

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