GSM6234767: Ribo-seq SKBR3 cells, VIRMA-overexpressing replicate 3; H... - SRA

SRX15673738: GSM6234767: Ribo-seq SKBR3 cells, VIRMA-overexpressing replicate 3; Homo sapiens; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 24.7M spots, 7.4G bases, 2.2Gb downloads

External Id: GSM6234767_r1

Submitted by: Epigenetics and RNA Biology Program, Centenary Institute

Study: Study the global mRNA translation changes between SKBR cells overexpressing VIRMA and eGFP control

PRJNA847982 • SRP379598 • All experiments • All runs

show Abstracthide Abstract

In order to determine whether increased m6A abundance coupled with upregulation of transcripts regulating UPR also promote their translation, we first compared levels of key UPR signalling proteins in VIRMA FL-overexpressing SKBR3 cells and eGFP control. To our surprise, none of the key UPR signalling proteins measured showed increased levels in VIRMA FL cells compared to control We then performed ribosome profiling analysis between SKBR cells overexpressing VIRMA FL and eGFP control Overall design: Comparative translation analysis of Poly-ribo-seq data for VIRMA-overexpressing SKBR3 cells and eGFP control

Sample: Ribo-seq SKBR3 cells, VIRMA-overexpressing replicate 3

SAMN28968546 • SRS13371927 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: GSM6234767

Instrument: Illumina HiSeq 2500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Construction protocol: Polysome fractions were collected using an LC-MS/MS-based ribo Mega-Sec approach as previously described. Total RNA was extracted from the eluted polysome fractions using TRIzol LS. Ribosomal RNA depleted library 1 μg total RNA using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Kit (Illumina), according to the manufacturers' instructions. A total of 25 million 150 bp paired-end strand-specific reads were sequenced per sample on an Illumina HiSeq® 2500 Platform (Illumina).

Runs: 1 run, 24.7M spots, 7.4G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR19623110	24,714,384	7.4G	2.2Gb	2023-08-03

ID:: 22297261

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity