RNA-seq of Neocallimastix frontalis strain EC30 - SRA

SRX15663492: RNA-seq of Neocallimastix frontalis strain EC30
1 ILLUMINA (Illumina HiSeq 1500) run: 22.5M spots, 6.7G bases, 2Gb downloads

Design: Total RNA was harvested from the growing strains isolated that had been maintained under anaerobic conditions initially isolated by a modified Hungate method. Enrichment of mRNA was performed by oligo(dT) beads. The mRNA is then fragmented randomly by fragmentation buffer, and cDNA synthesized from mRNA template and random hexamers primers. A custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. After a series of terminal repairs, A ligation, and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.

Submitted by: Oklahoma State University

Study: Anaerobic fungal strains transcriptomic datasets

PRJNA847081 • SRP379492 • All experiments • All runs

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Transcriptomic datasets from 20 anaerobic fungal isolates belonging to 13 genera

Sample:

SAMN28920474 • SRS13361876 • All experiments • All runs

Organism: Neocallimastix frontalis

Library:

Name: EC30

Instrument: Illumina HiSeq 1500

Strategy: RNA-Seq