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SRX15281717: GSM6153507: CKO male2, Riboseq; Mus musculus; OTHER
2 ILLUMINA (Illumina NovaSeq 6000) runs: 138M spots, 10.5G bases, 2.8Gb downloads

External Id: GSM6153507_r1
Submitted by: Molecular Genetics and Microbiology, Duke University School of Medicine
Study: Identification of DDX3X-dependent translation targets in the E11.5 mouse cortex
show Abstracthide Abstract
To identify DDX3X-dependent translation targets in the developing mouse cortex, we used E11.5 cortices from both sexes of wildtype and conditional Ddx3x knockout mice (driven by Emx1-Cre). We performed RNAseq and Riboseq on these samples in parallel. Overall design: Comparative gene expression profiling analysis of RNA-seq and Ribo-seq data for wildtype and Ddx3x conditional knockout mouse E11.5 cortex. The following are biological replicates: Female controls: F2, F3; Male controls: M1, M2, M3; cKO females: F1, F2, F3; cKO mals: M1, M2, M3
Sample: CKO male2, Riboseq
SAMN28447080 • SRS13013117 • All experiments • All runs
Organism: Mus musculus
Library:
Name: GSM6153507
Instrument: Illumina NovaSeq 6000
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: RNA-seq: RNA was harvested using the RNAeasy kit (Qiagen, 74034). 50 ng of total RNA was used for the construction of sequencing libraries. Riboseq: Ribosome protected fragments were isolated on a 15% denaturing urea gel followed by gel extraction and Trizol-based RNA isolation. RNAseq: cDNA libraries for RNA-seq were prepared using the Kapa mRNA HyperPrep kit with mRNA capture (KapaBiosystems, KR1352) Riboseq: cDNA libraries for Ribo-seq were prepared using the Qiagen miRNA library prep kit (331502)
Runs: 2 runs, 138M spots, 10.5G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
SRR1921771068,731,9965.2G1.4Gb2022-05-18
SRR1921771169,238,1265.3G1.4Gb2022-05-18

ID:
21813042

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