GSM4886543: sgDROSHA Ribosome profiling Rep2; Homo sapiens; OTHER - SRA

SRX9444529: GSM4886543: sgDROSHA Ribosome profiling Rep2; Homo sapiens; OTHER
1 ILLUMINA (Illumina HiSeq 2500) run: 8.3M spots, 244.5M bases, 125Mb downloads

Submitted by: NCBI (GEO)

Study: Ribosome profiling and mRNA-seq of muscle skeletal muscle and DROSHA KO 293T cells

PRJNA674837 • SRP291315 • All experiments • All runs

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We conducted ribosome profiling and mRNA-seq using mouse skeletal muscle tissues and 293T cells (NTC control and DROSHA KO) with Hiseq 2500 Overall design: Examination of translation status of mouse skeletal muscle and the differences between NTC and DROSHA KO in 293T cells

Sample: sgDROSHA Ribosome profiling Rep2

SAMN16676459 • SRS7658454 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: OTHER

Source: TRANSCRIPTOMIC

Selection: other

Layout: SINGLE

Construction protocol: Both skeletal muscle and cells were lysed with polysome lysis buffer TruSeq Ribo Profile (Mammalian) Kit (illumina) was used with some modifications. For mRNA-Seq, ploA mRNA was purified before library construction.

Experiment attributes:

GEO Accession: GSM4886543

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 8.3M spots, 244.5M bases, 125Mb

Run	# of Spots	# of Bases	Size	Published
SRR12993029	8,279,734	244.5M	125Mb	2021-01-01

ID:: 12341090

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