Sff_Glu - SRA

SRX7957253: Sff_Glu
1 ILLUMINA (Illumina MiSeq) run: 165,643 spots, 99.7M bases, 49.5Mb downloads

Design: The flower and pollen samples were stored in a portable refrigerator after collection and transported to the laboratory, where each sample was weighed and adjusted to 1 g. For flower samples, 30 mL of 1 X PBS buffer (10 X PBS: 8 g of NaCl, 0.2 g of KCl, 1.44 g of Na2HPO4, 0.24 g of KH2PO4, adjusted to a pH of 7.4 and a final volume of 1 L) was added. Pollen samples received 20 mL of 1 X PBS buffer. Then the sample tubes were sonicated at 35 kHz (20 min, 4C) to detach microbes and 3 mL of the mixture was centrifuged (12,470 x g, 10 min, 4C). The supernatant was discarded and total DNA in the pellet was purified by using a Genomic DNA isolation kit (Solgent, Korea).

Submitted by: Gyeongsang national university

Study: Streptomyces flower Glu

PRJNA613305 • SRP253410 • All experiments • All runs

show Abstracthide Abstract

Why Strawberry anthosphere microbiome structure is collapse at unhealthy plant ?

Sample: Metagenome or environmental sample from flower metagenome

SAMN14400506 • SRS6343358 • All experiments • All runs

Organism: flower metagenome

Library:

Name: 1G1

Instrument: Illumina MiSeq

Strategy: OTHER