show Abstracthide Abstract<i><B>Escherichia coli</i> strain K-12 substrain MG1655</B>. <I>E. coli</I> K-12 holds a key position as a model organism in studies of molecular biology, biochemistry, genetics and biotechnology. A high-quality reference sequence of the genome of E. coli K-12 strain MG1655 has previously been generated using the Sanger dideoxy sequencing method (Reference sequence accession: NC_000913). This genome of the MG1655 strain is being sequenced using the Solexa sequencing method on the Illumina Genome Analyzer. The purpose of this project was to provide a resource of paired-end, short-read sequence data from a relatively small genome for development of de novo sequence assembly algorithms or other analysis tools.