RNA-seq of pooled gill tissue samples from 12 walleye - SRA

SRX4221801: RNA-seq of pooled gill tissue samples from 12 walleye
1 ILLUMINA (Illumina HiSeq 4000) run: 326.8M spots, 65.4G bases, 26.9Gb downloads

Design: Total RNA was extracted from gill tissues using RNeasy Plus Mini Kits (Qiagen). Concentration and integrity of the RNA were assessed using a Nanodrop One Spectrophotometer, a Qubit, and gel electrophoresis. Two random samples were chosen from six sampling locations (n = 12 samples) and equal amounts of total RNA (2000ng) were pooled. The quality of the pooled sampled was assessed using a Bioanalyzer (Agilent) and had a RIN of 8.6. The pooled sample was used for a stranded mRNA isolation library preparation using a NEBnext library prep kit. Sequencing was conducted to produce 100 paired-end reads over one Illumina HiSeq 4000 lane.

Submitted by: University of Manitoba

Study: Generating a Reference Transcriptome for Walleye

PRJNA476338 • SRP150633 • All experiments • All runs

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Walleye (Sander vitreus) are an important commercial and recreational fish species in North America. Lake Winnipeg, Manitoba, supports the second largest freshwater fishery in Canada, with walleye being a focal species. In 2017, wild-caught walleye from six different locations in the Lake Winnipeg and Lake Manitoba basins were collected and sampled for gill tissue. One of the goals of a larger project examining movement patterns in Lake Winnipeg is to develop molecular tools for examining the effects of environmental stressors on walleye in Manitoba. We used gill tissue samples from individuals from the different sampling locations to develop a reference transcriptome for future studies examining the effects of environmental stressors on this species.

Sample: Pooled sample of total RNA from 12 individuals from 6 locations

SAMN09430511 • SRS3422459 • All experiments • All runs

Organism: Sander vitreus

Library:

Name: Walleye_Pooled

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 326.8M spots, 65.4G bases, 26.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR7348420	326,849,617	65.4G	26.9Gb	2019-07-01

ID:: 5708788

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