RNA-Seq of Lagenaria siceraria: leaf - SRA

SRX13558024: RNA-Seq of Lagenaria siceraria: leaf
1 ILLUMINA (Illumina HiSeq 2000) run: 24.7M spots, 7.4G bases, 2.1Gb downloads

Design: The mRNA is interrupted by ultrasound by an ultrasonic wavelength mRNA with an ultrasonic wave.The fragment-based mRNA is analog, and the random oligonucleotide is primer, and the CDNA first strand is synthesized in the M-Mulv reverse transcriptase system, followed by RNaseH to degrade the RNA chain, and under the DNA Polymerase I system, DNTPS isThe raw material synthesis cDNA second chain.The purified double-stranded CDNA passes through the end repair, plus A tail and connects to the sequencing connector, and uses the CDNA of 200 bp with Ampure XP Beads, and the PCR amplification is performed and the PCR product is used to purify the PCR product again, and finally obtain the library.

Submitted by: Zhejiang Academy of Agriculture Science

Study: Transcriptome sequencing of Lagenaria siceraria

PRJNA793252 • SRP352949 • All experiments • All runs

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Two bottle gourd varieties (cv. J83 and J73) which have inequable PM resistance were used.

Sample:

SAMN24525483 • SRS11450953 • All experiments • All runs

Organism: Lagenaria siceraria

Library:

Name: J83-CK biological replicate 1

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq