GAME57 - SRA

SRX10573887: GAME57
1 ILLUMINA (Illumina MiSeq) run: 13,090 spots, 7.9M bases, 3.5Mb downloads

Design: Briefly, the DNA of the cooled (4C) samples was extracted using heat-lysis approach with an alkaline buffer. After quality controls of the extracted DNA, the V3-V4 variable regions of the 16S rRNA gene were amplified in a one-step PCR (30x cycles: 98 C/30 s - 98 C/9 s - 55 C/1 min - 72 C/1.30 min - 72 C/10 min - hold at 4C) using the primer pair 341F-806R. After verification of the presence of PCR-products by gel electrophoresis, normalisation (SequalPrep Normalisation Plate Kit; ThermoFisher Scientific, Waltham, USA) and equimolar pooling was performed. Sequencing was conducted on the MiSeq platform (MiSeqFGx; Illumina, San Diego, USA) with v3 chemistry.

Submitted by: GEOMAR Helmholtz Centre for Ocean Research Kiel

Study: Plastisphere amplicon reads from four different coastal locations from four different plastic types

PRJNA720815 • SRP314334 • All experiments • All runs

show Abstracthide Abstract

In this study, 16S rRNA gene amplicon data (V3-V4 region) was utilized to obtain first insights into the bacterial colonization patterns on various synthetic polymer types: high-density polyethylene (HDPE), linear low-density polyethylene (LDPE), polyamide (PA), polymethyl methacrylate (PMMA) incubated for five weeks in the upper one meter of the coastal water column in four distinct locations (Cape Verde, Chile, Japan, South Africa).

Sample: GAME57

SAMN18680277 • SRS8678523 • All experiments • All runs

Organism: biofilm metagenome

Library:

Name: I33235-377

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 13,090 spots, 7.9M bases, 3.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR14207132	13,090	7.9M	3.5Mb	2021-04-21

ID:: 14049481

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity