SRX7964233: RNA-seq of Cucumis sativus: fruit
1 ILLUMINA (HiSeq X Ten) run: 23.7M spots, 7.1G bases, 2.2Gb downloads
Design: The libraries was constructed according to the method of Qi et al. (2010). The clean reads were mapped to the reference Cucumber ChineseLongv3 Genome (http://cucurbitgenomics.org/organism/20) using Bowtie8 or Tophat (http://tophat.cbcb.umd.edu/). The fragments per kilo base of transcript per million fragments mapped (FPKM) values of each gene were calculated using Cufflinks, and the read counts of each gene were obtained by htseq-count. At least a 1.5-fold change in expression and a P-value <0.05 were set for identifying differentially expressed genes (DEGs). All DEGs were mapped to terms in the KEGG database to identify enriched pathway.
Submitted by: Yangzhou University
Library:
Name: Fruit-NP 1
Instrument: HiSeq X Ten
Strategy: RNA-Seq
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 23.7M spots, 7.1G bases, 2.2Gb