454 sequencing of Eucalyptus grandis transcript fragment library - SRA

SRX000427: 454 sequencing of Eucalyptus grandis transcript fragment library
4 LS454 (454 GS 20) runs: 1M spots, 166.4M bases, 402.2Mb downloads

Design: Three greenhouse-grown Eucalyptus grandis seedlings from each of seven open-pollinated families (21 genotypes in total) were dissected into xylem (Xy), phloem (Ph), roots (R), young leaves(YL), mature leaves (ML) and apical/lateral meristems (M). Tissues were immediately frozen in liquid nitrogen and stored. For each tissue, RNA was extracted from a pool of equal proportion of plant material from all 21 genotypes. RNA isolated from each tissue pool was combined in varying proportions (10% YL, 10% ML, 15% R, 15% Ph, 20% M and 30% Xy) to a single pool. cDNA was synthesized with Clontech SMART cDNA Library Construction Kit (Qiagen USA, Valencia, CA) and normalized with Evrogen Trimmer-Direct Kit (Evrogen, Moscow).

Submitted by: Forest Genomics Lab - University of Florida (FORGEN)

Study: SNP discovery in E. grandis via a multi-genotype cDNA library

PRJNA30415 • SRP000218 • All experiments • All runs

Sample: Generic sample from Eucalyptus grandis

SAMN00000301 • SRS000519 • All experiments • All runs

Organism: Eucalyptus grandis

Library:

Name: E. grandis multi-genotype cDNA library

Instrument: 454 GS 20

Strategy: EST

Source: TRANSCRIPTOMIC

Selection: other

Layout: SINGLE

Spot descriptor:

5 Adapter

Runs: 4 runs, 1M spots, 166.4M bases, 402.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR001658	15,236	1.7M	4.1Mb	2008-07-10
SRR001659	303,149	33.4M	79.5Mb	2008-07-10
SRR001660	328,486	37.5M	87.8Mb	2008-07-10
SRR001661	395,005	93.8M	230.8Mb	2008-07-10

ID:: 428

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity