assay type:
transcription profiling by high throughput sequenc (show full text...)(hide...)transcription profiling by high throughput sequencing
sample storage: RNAlater, frozen
sample storage processing: cryopreservation, other
sampling to preparation interval: 295 days
experimental protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
extraction protocol:
ftp://ftp.faang.ebi.ac.uk/ftp/protocols/samples/RO (show full text...)(hide...)ftp://ftp.faang.ebi.ac.uk/ftp/protocols/samples/ROSLIN_SOP_Isolation_of_RNA_from_RNAlater_preserved_tissue_20161108.pdf
library preparation location:
Edinburgh Genomics, The University of Edinburgh, E (show full text...)(hide...)Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
library preparation location longitude: -3.173114 decimal degrees
library preparation location latitude: 55.923953 decimal degrees
library preparation date: 2015-10 YYYY-MM
sequencing location:
Edinburgh Genomics, The University of Edinburgh, E (show full text...)(hide...)Edinburgh Genomics, The University of Edinburgh, EH9 3FL, Edinburgh, Scotland
sequencing location longitude: -3.173114 decimal degrees
sequencing location latitude: 55.923953 decimal degrees
sequencing date: not applicable
experiment target: polyA RNA
rna preparation 3' adapter ligation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
rna preparation 5' adapter ligation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation pcr product isolation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
preparation reverse transcription protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
library generation protocol:
https://support.illumina.com/content/dam/illumina- (show full text...)(hide...)https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqstrandedmrna/truseq-stranded-mrna-sample-prep-guide-15031047-e.pdf
read strand: mate 2 sense
rna purity - 260:280 ratio: 2.09
rna purity - 260:230 ratio: 2.25
rna integrity number: 7.4