Name: HGWPI
Instrument: Illumina Genome Analyzer II
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Construction protocol: A total of 5 μg of genomic DNA from HCC1954 and HCC1954BL cell lines was randomly sheared using a Biorupter according to the manufacturer’s instructions. The fragmented DNA was end-repaired using Klenow and T4 DNA polymerases and phosphorylated at the 5’ end with T4 polynucleotide kinase. A 3’ overhang was created using 3’-5’ exonuclease-deficient Klenow fragment and Illumina paired-end adaptor oligonucleotides were ligated to the created sticky ends. DNA fragments of ~200bp were size selected in 8% polyacrylamide gels and eluted from the gel overnight. Size-selected DNA was PCR amplified for 18 cycles to enrich for adapter-modified DNA fragments. A paired-end flow cell was prepared on the supplied cluster station according to the manufacturer’s protocol. Clusters of PCR colonies were then sequenced on the Illumina GAII sequencing platform using the recommended protocols. Images from the instrument were processed to generate FASTQ sequence files.