MinION sequencing of SAMD00127215 - SRA

DRX131256: MinION sequencing of SAMD00127215
1 OXFORD_NANOPORE (MinION) run: 714,931 spots, 984.5M bases, 822.3Mb downloads

Submitted by: University of Tokyo, Medical Genome Sciences (UT-MGS)

Study: Transcriptome sequencing using nanopore sequencing

PRJDB7059 • DRP004517 • All experiments • All runs

show Abstracthide Abstract

For general RNA-Seq using short read sequencer, it is difficult to reveal entire structure of transcripts. To overcome this problem, we performed the MinION sequencing of full-length cDNA synthesized by SMART-Seq v4. We applied these data to gene expression estimation, detection of fusion genes, and SNP phasing.

Sample: FL-cDNA-Seq of skeletal muscle of a male (R9.4)

SAMD00127215 • DRS076414 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: Male_Skeletal_muscle_cDNA

Instrument: MinION

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: The full-length cDNA was synthesized from 50 ng of total RNA and SMART-Seq v4 kit. The 2D sequnencing library was constructed from 1 u g of full-length cDNA by 2D Sequencing Kit (SQK-LSK208).

Spot descriptor:

1 forward

Runs: 1 run, 714,931 spots, 984.5M bases, 822.3Mb

Run	# of Spots	# of Bases	Size	Published
DRR138518	714,931	984.5M	822.3Mb	2018-10-13

ID:: 6576518

SRA

Sequence Read Archive

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