MinION sequencing of SAMD00127207 - SRA

DRX131248: MinION sequencing of SAMD00127207
1 OXFORD_NANOPORE (MinION) run: 46,672 spots, 42.8M bases, 36Mb downloads

Submitted by: University of Tokyo, Medical Genome Sciences (UT-MGS)

Study: Transcriptome sequencing using nanopore sequencing

PRJDB7059 • DRP004517 • All experiments • All runs

show Abstracthide Abstract

For general RNA-Seq using short read sequencer, it is difficult to reveal entire structure of transcripts. To overcome this problem, we performed the MinION sequencing of full-length cDNA synthesized by SMART-Seq v4. We applied these data to gene expression estimation, detection of fusion genes, and SNP phasing.

Sample: FL-cDNA-Seq of VMRC-LCD (R9)

SAMD00127207 • DRS076406 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: VMRCLCD_cDNA_R9

Instrument: MinION

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: The full-length cDNA was synthesized from 50 ng of total RNA and SMART-Seq v4 kit. The 2D sequnencing library was constructed from 1 u g of full-length cDNA by 2D Sequencing Kit (SQK-MAP007).

Spot descriptor:

1 forward

Runs: 1 run, 46,672 spots, 42.8M bases, 36Mb

Run	# of Spots	# of Bases	Size	Published
DRR138510	46,672	42.8M	36Mb	2018-10-13

ID:: 6576510

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Sequence Read Archive

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