show Abstracthide AbstractDifferent plant lineages produce distinct types of alkaloids, implying tradeoffs during the evolution of these defensive compounds. Tribes in the Solanaceae plant family produce varying alkaloids, such as tropane alkaloids (TAs) and steroidal glycoalkaloids (SGAs). We conducted a search for regulatory networks associated with this metabolic divergence by integrating transcriptomic and metabolomic variation across numerous Solanaceae species.