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SRX6080428: Illumina-seq of ciliate: V9 region of 18S rDNA
1 ILLUMINA (NextSeq 500) run: 1.1M spots, 323.8M bases, 118.3Mb downloads

Design: DNA extraction with Qiagens DNeasy Blood & Tissue Kit. PCR amplification with V9 specific primers (V9F: 5-GTACACACCGCCCGTC-3; Lane, 1991; V9R: 5-TGATCCTTCTGCAGGTTCACCTAC-3; Medlin et al., 1988). Sequencing library construction for Illumina sequencing with respective Illumina kit.
Submitted by: University of Kaiserslautern
Study: Illumina-sequencing of 29 individual ciliate species
PRJNA548847 • SRP201658 • All experiments • All runs
show Abstracthide Abstract
The V9 region of the 18S rRNA gene of 29 individual ciliate species was amplified and Illumina-sequenced to compare the performance of a recently developed ASV method (DADA2) against a popular local OTU clustering algorithm (SWARM).
Sample:
SAMN12057300 • SRS4982261 • All experiments • All runs
Organism: Coleps hirtus hirtus
Library:
Name: Cil28
Instrument: NextSeq 500
Strategy: AMPLICON
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 1.1M spots, 323.8M bases, 118.3Mb
Run# of Spots# of BasesSizePublished
SRR93128031,115,097323.8M118.3Mb2019-08-19

ID:
8131620

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