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SRX3442529: RNA-Seq of Helgoeca nana ATCC 50073
1 ILLUMINA (Illumina HiSeq 2000) run: 58.7M spots, 11.7G bases, 7.5Gb downloads

Design: RNA isolation with RNAqueous kit, genomic DNA digestion with TURBO DNA-free kit, library preparation with Illumina TruSeq v2 mRNA kit, modified to add two extra rounds of polyA mRNA selection (four rounds total)
Submitted by: University of California, Berkeley
Study: Transcriptome sequencing of 19 diverse species of choanoflagellates
show Abstracthide Abstract
The origin of animals, which occurred over 600 million years ago, left no evidence in the fossil record. To trace the earliest events in animal prehistory, we compare extant animals to their closest living relatives, the choanoflagellates, in order to reconstruct the gene content of their last common ancestor and how it evolved on the stem lineage leading to animals. In this project, we increase the accuracy of ancestral animal and choanoflagellate gene content reconstructions by sequencing the transcriptomes of 19 species of choanoflagellates selected for their phylogenetic diversity.
SAMN08053108 • SRS2733210 • All experiments • All runs
Organism: Helgoeca nana
Name: 08
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 58.7M spots, 11.7G bases, 7.5Gb
Run# of Spots# of BasesSizePublished


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