target enrichment of UCEs and exons from anthozoans - SRA

SRX7138567: target enrichment of UCEs and exons from anthozoans
1 ILLUMINA (Illumina HiSeq 3000) run: 2.1M spots, 639M bases, 252Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit with dual indexed iTru adapters (Glenn et al. 2016). The resulting DNA libraries were combined in equimolar pools of 5 to 8 samples. Pooled libraries were enriched for UCE loci using the anthozoa-v1 bait set (Quattrini et al. 2017) synthesized by MYcroarray (Ann Arbor, MI). We quantified each enriched pool, post- PCR amplification, using a Qubit fluorometer and checked them for adapter contamination using a Bioanalyzer. If adapters were found, a 1X bead cleanup was performed. After cleaning up any remaining adapter contamination, we qPCR quantified each pooled library using the Kapa qPCR quantification kit, and we combined pooled libraries prior to sequencing.

Submitted by: Harvey Mudd College

Study: Phylogenomics of Anthozoa: New Approaches to long-standing problems

PRJNA588468 • SRP229811 • All experiments • All runs

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Sequence data were captured using a targeted enrichment approach for UCEs and exons.

Sample:

SAMN13244951 • SRS5647236 • All experiments • All runs

Organism: Sagartia troglodytes

Library:

Name: ANT219

Instrument: Illumina HiSeq 3000

Strategy: WGS

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 2.1M spots, 639M bases, 252Mb

Run	# of Spots	# of Bases	Size	Published
SRR10443659	2,115,922	639M	252Mb	2020-05-01

ID:: 9383510

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