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SRX17171527: Hyb-Seq data of 33 species of the plant family lonicera and three species from closely related family Targeted loci
1 ILLUMINA (Illumina HiSeq 2000) run: 6.3M spots, 1.9G bases, 720.1Mb downloads

Design: DNA was extracted using either a DNeasy kit (Qiagen, Valencia, California, USA) or with a modified 2 CTAB method (Doyle and Doyle 1987). Genomic DNAs were sheared by nebulization at 30 psi for 70 sec, yielding an average shear size of 500bp as measured by a Bioanalyzer High-Sensitivity Chip (Agilent Technologies, Inc., Santa Clara, California, USA). Libraries were constructed using the Illumina TruSeq library preparation kit (Illumina Inc., San Diego, California, USA) and NEXTflex DNA barcodes (Bioo Scientific, Austin, Texas, USA), standardized at 2nM, and pooled in 16-plexes prior to hybrid enrichment. Solution-based hybridization with MYbaits biotinylated RNA baits (MYcroarray, Ann Arbor, Michigan, USA) and enrichment followed Weitemier et al. (2014). The target-enriched libraries were then sequenced on an Illumina HiSeq 2000 with 150bp paired-end reads.
Submitted by: Hainan university
Study: Target sequence capture data shed light on the deeper evolutionary relationship on the subgenus Chamaecerasus of Lonicera (Caprifoliaceae)
show Abstracthide Abstract
The genus Lonicera L. is widely distributed and is well-known for its high species richness and morphological diversity. Previous studies have suggested that many sections of Lonicera are not monophyletic and phylogenetic relationships within the genus are still poorly known.
SAMN30276312 • SRS14741734 • All experiments • All runs
Name: Lonicera_caprifolium_G25
Instrument: Illumina HiSeq 2000
Strategy: Targeted-Capture
Selection: Hybrid Selection
Layout: SINGLE
Runs: 1 run, 6.3M spots, 1.9G bases, 720.1Mb
Run# of Spots# of BasesSizePublished


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