mRNAseq of Varanus beccarii: adult female blood - SRA

SRX5273405: mRNAseq of Varanus beccarii: adult female blood
1 ILLUMINA (NextSeq 500) run: 40.3M spots, 3.4G bases, 1.2Gb downloads

Design: Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturers protocol from blood samples of three females and two males of Varanus komodoensis, a female of V. macraei and a female of V. beccari. Libraries were constructed by GeneCore (EMBL, Heidelberg, Germany). The barcoded stranded mRNA-sequencing libraries were prepared using the Illumina TruSeq mRNA v2 sample preparation kit (Illumina, San Diego, CA, USA) with poly-A mRNA enrichment, implemented on the liquid handling robot Beckman FXP2. The resulting libraries were pooled together and loaded on the Illumina NextSeq 500 sequencer and 84 base pairs (bp) were sequenced unidirectionally.

Submitted by: Faculty of Science, Charles University

Study: Evolution of sex chromosomes and dosage compensation in monitor lizards

PRJNA516155 • SRP180989 • All experiments • All runs

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Sex determination in varanids, Gila monsters, beaded lizards and other anguimorphan lizards is still poorly understood. Sex chromosomes were reported only in a few species based solely on cytogenetics, which precluded assessment of their homology. We uncovered Z-chromosome specific genes in varanids from their transcriptomes. The analysis of transcriptomes of Komodo dragon (Varanus komodoensis) showed that the expression levels of genes linked to anguimorphan sex chromosomes are not balanced between sexes and hence lack global dosage compensation.

Sample:

SAMN10782977 • SRS4273735 • All experiments • All runs

Organism: Varanus beccarii

Library:

Name: VABE_F_library

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: SINGLE

Runs: 1 run, 40.3M spots, 3.4G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR8466821	40,255,642	3.4G	1.2Gb	2020-02-01

ID:: 7110081

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