DNA Seq of Enterocloster sp005845215 OA11 - SRA

SRX14032919: DNA Seq of Enterocloster sp005845215 OA11
1 PACBIO_SMRT (Sequel) run: 733,204 spots, 3.4G bases, 825.2Mb downloads

Design: Extracted DNA from bacterial isolates was sheared to an average size of 13,000 bp. Each sheared sample was barcoded with the Pacbio barcode overhang adapter kit 8A (cat#101-628-400) during library prep using the Pacbio SMRTbell Express Template Prep kit 2.0 (cat#100-938-900). Samples were combined to an equal concentration using the Pacbio Express microbial multiplexing calculator to give an estimated size of about 30mb total. The Pacbio Sequencing Primer v4 was conditioned and annealed to the pooled sample based on the Diffusion loading instructions found on the SMRT link pacbio software version 8.0.0805529. Following primer binding, the diluted Pacbio Sequel Polymerase 3.0 (cat#100-970-100) was bound to the sample. After binding, the sample was cleaned with Pacbio Ampure Beads (cat#100-265-900) and eluted in Pacbio Elution Buffer (cat#101633-500).

Submitted by: New York University

Study: Clostridia and Bacilli isolated from helminth-colonized humans

PRJNA800461 • SRP358106 • All experiments • All runs

show Abstracthide Abstract

Although helminth colonization has been associated with altered composition of the gut microbiota, such as increases in Clostridia, individual species have not been isolated and characterized. Here, we isolated and sequenced the genome of 14 Firmicutes from the Orang Asli, an indigenous population in Malaysia with high prevalence of helminth infections.

Sample:

SAMN25248159 • SRS11868076 • All experiments • All runs

Organism: Enterocloster sp. OA11

Library:

Name: r54195_20210224_194310-3

Instrument: Sequel

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Runs: 1 run, 733,204 spots, 3.4G bases, 825.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR17873549	733,204	3.4G	825.2Mb	2022-02-09

ID:: 19767381

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