DNA-seq of Ipomoea sp - SRA

SRX13778828: DNA-seq of Ipomoea sp
1 ILLUMINA (Illumina NovaSeq 6000) run: 145M spots, 43.5G bases, 12.8Gb downloads

Design: The genomic DNA was randomly fragmented by sonication, then DNA fragments were end polished, A-tailed, and ligated with the full-length adapters of Illumina sequencing, and followed by further PCR amplification with P5 and indexed P7 oligos. The PCR products as the final construction of the libraries were purified with AMPure XP system. Then libraries were checked for size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA), and quantified by real-time PCR (to meet the criteria of 3 nM). The qualified libraries are fed into Illumina sequencers after pooling according to its effective concentration and expected data volume.

Submitted by: University of Oxford

Study: Discovery and characterization of sweetpotato's closest tetraploid relative

PRJNA796763 • SRP354967 • All experiments • All runs

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In this study, we identified wild autotetraploid plants from Ecuador that are morphologically distinct from Ipomoea batatas and Ipomoea trifida, but monophyletic and sister to Ipomoea batatas in phylogenetic analysis of nuclear data. We describe them as a new species, Ipomoea aequatoriensis T.Wells & P.Munoz. This species is the sweetpotato's closest tetraploid wild relative and likely played a direct role in the origin of sweetpotato's hexaploid genome.

Sample:

SAMN24913461 • SRS11661602 • All experiments • All runs

Organism: Ipomoea sp. CH80.3

Library:

Name: CH80.3

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 145M spots, 43.5G bases, 12.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR17610135	145,031,821	43.5G	12.8Gb	2022-01-24

ID:: 19171062

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