Ultraconserved element target capture of Cletus sp. - SRA

SRX7826335: Ultraconserved element target capture of Cletus sp.
1 ILLUMINA (Illumina HiSeq 3000) run: 3.2M spots, 639.8M bases, 269.8Mb downloads

Design: Libraries were constructed from genomic DNA (sheared to 200 to 1000 bp) using a KAPA Hyper Prep Kit with half volume reactions, iTru universal adapter stubs, and iTru 8 bp dual-indexes (Glenn et al., 2016). Hydrophobic Sera-Mag SpeedBeads Carboxyl Magnetic Beads were used for all cleanup steps. Cleaned, amplified libraries were quantified with Qubit, subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) was used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. Probes were hybridized with library pools at 65C for 18 hours, followed by 18 hours at 62C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 62C, and resuspended in 30 uL IDTE. We used 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14 to 17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Floridas Interdisciplinary Center for Biotechnology Research (ICBR).

Submitted by: University of Florida

Study: The evolution of autotomy in leaf-footed bugs

PRJNA609116 • SRP251224 • All experiments • All runs

show Abstracthide Abstract

Ultraconserved element loci used to generate a molecular phylogeny of leaf-footed bugs and for comparative analysis of limb-loss (autotomy).

Sample:

SAMN14229794 • SRS6237474 • All experiments • All runs

Organism: Cletus sp. CMF 0765

Library:

Name: Cletus sp. CMF0765

Instrument: Illumina HiSeq 3000

Strategy: Targeted-Capture

Source: GENOMIC

Selection: Reduced Representation

Layout: PAIRED

Runs: 1 run, 3.2M spots, 639.8M bases, 269.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR11213496	3,167,158	639.8M	269.8Mb	2021-03-01

ID:: 10240374

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity