Ultraconserved element target capture of Leptoglossus corculus - SRA

SRX7826341: Ultraconserved element target capture of Leptoglossus corculus
1 ILLUMINA (Illumina HiSeq 3000) run: 2.1M spots, 432.2M bases, 182.6Mb downloads

Design: Libraries were constructed from genomic DNA (sheared to 200 to 1000 bp) using a KAPA Hyper Prep Kit with half volume reactions, iTru universal adapter stubs, and iTru 8 bp dual-indexes (Glenn et al., 2016). Hydrophobic Sera-Mag SpeedBeads Carboxyl Magnetic Beads were used for all cleanup steps. Cleaned, amplified libraries were quantified with Qubit, subsequently combined in equimolar amounts into 1000 ng pools. A custom MYbaits kit containing a subset of Hemiptera UCE probes designed from pentatomomorphan species (Faircloth, 2017) was used for target enrichment. The hybridization mixture used half volume baits (2.75 uL) with 2.75 uL molecular-grade water. Probes were hybridized with library pools at 65C for 18 hours, followed by 18 hours at 62C. Bait-target hybrids were bound to Dynabeads M-280 Streptavidin beads, washed at 62C, and resuspended in 30 uL IDTE. We used 2.5 uL each of 5 uM iTru P5/P7 primers (Glenn et al., 2016) for the post-capture PCR amplification mix. 14 to 17 cycles of post-capture amplification were performed, followed by cleaning. Enriched library pools were pooled in equimolar amounts and sequenced using a single Illumina HiSeq3000 lane (2x100 run) at the University of Floridas Interdisciplinary Center for Biotechnology Research (ICBR).

Submitted by: University of Florida

Study: The evolution of autotomy in leaf-footed bugs

PRJNA609116 • SRP251224 • All experiments • All runs

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Ultraconserved element loci used to generate a molecular phylogeny of leaf-footed bugs and for comparative analysis of limb-loss (autotomy).

Sample:

SAMN14229799 • SRS6237480 • All experiments • All runs

Organism: Leptoglossus corculus

Library:

Name: Leptoglossus corculus CMF0785

Instrument: Illumina HiSeq 3000

Strategy: Targeted-Capture

Source: GENOMIC

Selection: Reduced Representation

Layout: PAIRED

Runs: 1 run, 2.1M spots, 432.2M bases, 182.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR11213490	2,139,832	432.2M	182.6Mb	2021-03-01

ID:: 10240380

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