Sinaechinocyamus mai gut transcriptome - SRA

SRX12419617: Sinaechinocyamus mai gut transcriptome
1 ILLUMINA (Illumina NovaSeq 6000) run: 64.4M spots, 13G bases, 3.8Gb downloads

Design: Tissue subsamples were chopped and preserved in RNAlater (Ambion) buffer solution for 1 day at 4C, followed by long-term storage at -80C until RNA extraction. Total RNA was extracted from 1.5 mm Triple-Pure High Impact Zirconium beads (Benchmark Scientific) in Trizol (Ambion) using Direct-zol RNA Miniprep Kit (Zymo Research) with in-column DNase I incubation. Total RNA concentration was estimated using Qubit RNA HS Assay Kit (Invitrogen), and quality assessed using either High Sensitivity RNA ScreenTape or RNA ScreenTape with an Agilent 4200 TapeStation. Mature mRNA was isolated from total RNA and libraries were prepared using KAPA mRNA HyperPrep Kit (KAPA Biosystems), targeting an insert size of approx. 500 base pairs, and using custom 10-nucleotide Illumina TruSeq style adapters. Post-amplification, DNA concentration was estimated using Qubit dsDNA BR Assay Kit (Invitrogen). Concentration, quality, and molecular weight distribution of libraries was assessed using Genomic DNA ScreenTape with an Agilent 4200 TapeStation. Ten libraries were sequenced on one lane of a multiplexed run using NovaSeq S4 platform with 100 bp paired-end reads at the IGM Genomics Center (University of California San Diego)

Submitted by: Yale University

Study: Phylotranscriptomics of Echinoidea

PRJNA767441 • SRP339571 • All experiments • All runs

show Abstracthide Abstract

The projects involves the sequencing of transcriptomes from a sample of highly-divergent echinoid lineages in order to provide a basis for a phylogenomic classification of the group

Sample: Sinaechinocyamus mai transcriptome

SAMN21905103 • SRS10387195 • All experiments • All runs

Organism: Sinaechinocyamus mai

Library:

Name: SMAI

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 64.4M spots, 13G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR16134557	64,447,580	13G	3.8Gb	2022-06-30

ID:: 16831088

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