B19L* - SRA

SRX21159335: B19L*
1 ILLUMINA (Illumina NovaSeq 6000) run: 150,788 spots, 67.7M bases, 21.7Mb downloads

Design: FastDNA SPIN Kit for soil (MP Biomedicals, Irvine, CA) was used for DNA extraction using approximately 0.5 g from each sample according to the manufacturers instructions. The quantity and quality of the extracted genomic DNA was determined using a NanoDrop spectrophotometer (NanoDrop Technologies and gel electrophoresis on 1,8% agarose). Extracted DNAs were stored at 20C until further use. The universal primers 515F and 806R, targeting the V4 region of the 16S rRNA gene were used (341F (5-GTGYCAGCMGCCGCGGTAA-3) and 802R (5-GGACTACNVGGGTWTCTA- 3)) (Yu et al., 2005). The PCR was performed in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 5 min, followed by 30 cycles of 94C for 30 sec, 55C for 30 sec and 72C for 1.5 min and a nal elongation step at 72C for 10 min. The expected-size PCR products were puried in 1,0% agarose gel using a commercial kit (ZR Zymoclean TM Gel DNA Recovery Kit, USA). Adapter and barcodes for Ion Torrent sequencing were included in the primers following a previous strategy (Claesson et al., 2009). Sequencing of the 16S rRNA gene amplicon libraries was carried out using the Illumina Novaseq6000 PE 250 in the genomic plataform at the Bymycell Inova Simples Ltda (Ribero Petro, So Paulo state, Brazil).

Submitted by: University of Sao Paulo

Study: Hydrogen Production in a Mesophilic and Termophilic Single-chambered Microbial Fuel Cell from Acidified Sugarcane Vinasse

PRJNA998359 • SRP451478 • All experiments • All runs

show Abstracthide Abstract

The production of green hydrogen is widely researched as path of world descarbonization. Microbial electrolysis cell (MEC) is a bioelectrochemical process that use microorganism as economic biocatalysts, and electrochemical techniques for manipulating its metabolism. This recent technology relies on the use of renewable sources for hydrogen production such as streams with high organic matter. Therefore, this studio was based on the assessment of a single-chambered MEC for hydrogen production from acidified sugarcane vinasse. Two MEC were carried out in batch mode, one at 37 grade C poised at 0.8 V, 1.0 V and 1.2 V and, another at 55 grade C poised at 0.8 V, 0.9 V and 1.1 V. Hydrogen production rates was evaluated in relation to the specific surface area (PSA) and useful volume (PVOL) of the cell, and Coulombic efficiency, cathodic hydrogen recovery and molar fraction of hydrogen. The best hydrogen production was reached at 55 grade C under 1.1 V with PSA of 55.4 L H2/m2 d, PVOL of 1.55 L H2/.Ld and 96% molar fraction of hydrogen. It was found that the partial pressure of hydrogen affect the hydrogen evolution, leading to changes in the microbial pathway such as elongation-chain reactions through hydrogen reuse. Thermoanaerobacterium and Caproiciproducens were the most abundant genera identified in MEC at 37 grade C and Moorella in MEC at 55 grade C.

Sample: B19L*

SAMN36702004 • SRS18421508 • All experiments • All runs

Organism: Caloribacterium sp.

Library:

Name: DD5

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 150,788 spots, 67.7M bases, 21.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR25424731	150,788	67.7M	21.7Mb	2023-07-26

ID:: 28594669

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity