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SRX5670775: UCE target enrichment of acomys_louisae_TK25598: tissue
1 ILLUMINA (Illumina HiSeq 3000) run: 3M spots, 914.8M bases, 401.5Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit for Illumina platforms with dual indexed iTru adapters (Glenn et al. 2016). We combined the resulting DNA libraries in equimolar pools of eight samples and enriched each pool for 5,060 UCE loci using the Tetrapods-UCE-5Kv1 probeset(Faircloth etal. 2012) sold byMYcroarray (Ann Arbor, MI). We determined the size distribution of enriched libraries with an Agilent Bioanalyzer and removed remaining adapter dimer from pools where it was present using a 0.8 bead cleanup. We then quantied the enriched libraries with a Qubit 2.0 uorometer and pooled them in equimolar ratios.
Submitted by: Louisiana State University
Study: A phylogenomic rodent tree reveals the repeated evolution of masseter architectures
show Abstracthide Abstract
Understanding the number of times a trait has evolved is a necessary foundation for comprehending the selective regimes, developmental constraints, and evolutionary successes of clades. Rodents make up over 40% of mammalian species and their success has been partially attributed to the increase in biting efficiency which results from a forward shift of one or two portions of the masseter muscle from the zygomatic arch onto the rostrum. This forward shift has occurred in three discrete ways, but the number of times it has occurred has never been explicitly tested. We estimated an ultrametric phylogeny using ultraconserved elements, the first to include all rodent families, and examined support for evolutionary relationships among the five rodent suborders. We then incorporated relevant fossils, fit models of character evolution, and used stochastic character mapping to determine that a portion of the masseter muscle has moved forward onto the rostrum at least seven times, with one reversal during the ca. 70 MY history of rodents. The repeated evolution of this key innovation, its increasing prevalence through time, and the species diversity of clades with this character underscore the adaptive value of improved biting efficiency.
SAMN10715112 • SRS4613631 • All experiments • All runs
Organism: Acomys louisae
Name: acomys_louisae_TK25598
Instrument: Illumina HiSeq 3000
Strategy: WGS
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 3M spots, 914.8M bases, 401.5Mb
Run# of Spots# of BasesSizePublished


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