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SRX5105305: Anchored Hybrid Enrichment of Leptospermum macrocarpum: adult leaf
1 ILLUMINA (Illumina HiSeq 2500) run: 5.8M spots, 1.7G bases, 904.2Mb downloads

Design: Extracted genomic DNA was sheared on Covaris E220 Focused ultrasonicator to a fragment size of 300-800bp. Libraries were prepared and indexed (8 bp) on a Beckman-Coulter Biomek FXp liquid-handling robot. Hybrid-Enrichment was performed using the Angiosperm V1.0 kit. Samples were pooled equimolar for sequencing.
Submitted by: Florida State University
Study: The phylogenomics of diversification on an island: applying Anchored Hybrid Enrichment to New Zealand Leptospermum scoparium (Myrtaceae)
show Abstracthide Abstract
Leptospermum scoparium (Myrtaceae) is a morphologically highly variable species found in mainland Australia, Tasmania and New Zealand. In New Zealand up to six morphologically distinct varieties of this species have been described, although only two – var. scoparium and var. incanum – are widely recognised. In the present study we used Anchored Hybrid Enrichment to acquire sequence data from a ca. 485 orthologous low-copy nuclear loci for 27 New Zealand and three Australian accessions of L. scoparium as well as representatives of several other Leptospermum species. The final concatenated data matrix contained in excess of 32 × 106 nucleotide positions of which more than 53000 were parsimony informative. Phylogenetic trees constructed using different approaches differ in the level of resolution provided but together with a network analysis suggest broadly similar patterns of relationship. In particular, all our analyses are consistent with three geographically delimited groups within New Zealand L. scoparium. We discuss the implications of our results in terms of the evolution and intraspecific taxonomy of L. scoparium in New Zealand.
SAMN10528137 • SRS4114788 • All experiments • All runs
Name: I20880_25
Instrument: Illumina HiSeq 2500
Strategy: Targeted-Capture
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 5.8M spots, 1.7G bases, 904.2Mb
Run# of Spots# of BasesSizePublished


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