Nematogenys_inermis - SRA

SRX5635161: Nematogenys_inermis
1 ILLUMINA (Illumina HiSeq 2500) run: 2.2M spots, 423.9M bases, 152.1Mb downloads

Design: Library preparation and sequencing were performed at Arbor Biosciences (AB; arborbiosci.com; Ann Arbor, MI, USA). AB staff sheared 1-2g of DNA to 400-600 bps in length using a Diagenode Bioruptor Standard (UCD 200) with 6-8 cycles of sonication (depending on DNA quality) to prepare the libraries. The DNA libraries from 154 individuals were prepared using the Nextera (Epicentre Biotechnologies, Inc.) library preparation protocol for solution-based target enrichment following Faircloth et al. (2012) and increasing the number of PCR cycles following the tagmentation reaction to 20 as recommended by Faircloth et al (2013). AB staff used the Nextera library preparation protocol of in vitro transposition followed by PCR to prune the DNA and attach sequencing adapters (Adey et al. 2010)

Submitted by: State University of Sao Paulo

Study: Phylogenomic analysis of trichomycterids catfishes (Teleostei: Siluriformes) inferred from ultra conserved elements

PRJNA530617 • SRP190816 • All experiments • All runs

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Analyze the evolutionary relationships among the members of Trichomycetridae family

Sample:

SAMN11320952 • SRS4580934 • All experiments • All runs

Organism: Nematogenys inermis

Library:

Name: nematogenys_inermis3105

Instrument: Illumina HiSeq 2500

Strategy: Targeted-Capture

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 2.2M spots, 423.9M bases, 152.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR8847502	2,154,970	423.9M	152.1Mb	2019-12-31

ID:: 7596198

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