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SRX10914121: Mentzelia involucrata
1 ILLUMINA (NextSeq 500) run: 20.4M spots, 6.1G bases, 2.4Gb downloads

Design: DNA was isolated from samples, sheared to size of 550bp, libraries prepared using the KAPA LTP kit and then target enriched using Angiosperms353 probe set; 2*150 paired end
Submitted by: University of Georgia
Study: Cornales target enrichment using Angiosperms353
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Premise of the study: Cornales is an order of flowering plants containing ecologically and horticulturally important families, including Cornaceae (dogwoods) and Hydrangeaceae (hydrangeas), among others. While many relationships in Cornales are strongly supported by previous studies, some uncertainty remains with regards to the placement of Hydrostachyaceae, and relationships among families in Cornales and within Cornaceae. Here we analyze hundreds of nuclear loci to test published phylogenetic hypotheses and estimate a robust species tree for Cornales.Methods: Using the Angiosperms353 probe set and existing datasets, we generated phylogenomic data for 158 samples, representing all families in the Cornales, with intensive sampling in the Cornaceae.Key Results: We curated an average of 312 genes per sample, constructed maximum likelihood gene trees, and inferred a species tree using the summary approach implemented in ASTRAL-III, a method statistically consistent with the multispecies coalescent model.Conclusions: The species tree we constructed generally shows high support values and a high degree of concordance among individual nuclear gene trees. Relationships among families are largely congruent with previous molecular studies, except for the placement of the Nyssoids and the Grubbiaceae-Curtisiaceae clades. Furthermore, we were able to place Hydrostachyaceae within Cornales, and within Cornaceae, the monophyly of known morphogroups was well supported. However, patterns of gene tree discordance suggest potential ancient reticulation, gene flow and/or ILS in the Hydrostachyaceae lineage and the early diversification of Cornus. Our findings reveal new insights into the diversification process across Cornales and demonstrate the utility of the Angiosperms353 probe set.
Sample: Mentzelia involucrata
SAMN19110374 • SRS9004406 • All experiments • All runs
Name: 181
Instrument: NextSeq 500
Strategy: Targeted-Capture
Selection: size fractionation
Layout: PAIRED
Runs: 1 run, 20.4M spots, 6.1G bases, 2.4Gb
Run# of Spots# of BasesSizePublished


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