RNA-Seq of Pholoe baltica - SRA

SRX11582862: RNA-Seq of Pholoe baltica
1 ILLUMINA (Illumina HiSeq 4000) run: 32.5M spots, 6.5G bases, 2.7Gb downloads

Design: Tissue was either finely chopped, or small samples were placed as whole in RNAlater (Ambion) for 24 h at 4C and then stored at -80C or -20C. Total RNA was extracted from Trizol (Ambion) using Direct-zol RNA kits (Zymo Research) including a DNase I digest to remove genomic DNA following the manufacturers specifications. mRNA was purified with a Dynabeads mRNA Direct Micro Kit (Invitrogen). Quantity and quality of mRNA was assessed with a Qubit fluorometer (Invitrogen) and Tapestation RNA Screen Tape (Agilent). Libraries were prepared using KAPA Stranded mRNA-Seq kits (KAPA Biosystems) following the recommended protocol and using custom 10 nucleotide Illumina TruSeq style adapters. Libraries were sequenced on the Illumina HiSeq4000 using 150 base pairs paired end reads at the IGM Genomics Center (University of California San Diego).

Submitted by: UC San Diego

Study: Phylogenomics of Aciculata (Annelida)

PRJNA743301 • SRP330216 • All experiments • All runs

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Transcriptomes to assess the phylogenetic relationships within Aciculata (Annelida)

Sample:

SAMN20006046 • SRS9615708 • All experiments • All runs

Organism: Pholoe baltica

Library:

Name: ETPBA

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 32.5M spots, 6.5G bases, 2.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR15277956	32,503,017	6.5G	2.7Gb	2021-11-09

ID:: 15495631

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