Sinorhizobium phage vB_SmelM_phiM10 - SRA

SRX4645297: Sinorhizobium phage vB_SmelM_phiM10
1 ILLUMINA (Illumina MiSeq) run: 1.3M spots, 775.2M bases, 433.8Mb downloads

Design: Two separate phiM10 DNA samples, each from a plaque-purified phage sample, were sheared with a Diagenode Bioruptor. Indexed libraries were constructed with an NEBNext Ultra DNA Library Prep kit for Illumina (NEB, Ipswich, MA) in accordance with the manufacturers instructions. For each library, 1 g of DNA was end repaired and ligated to NEBNext adapters. Fragments of an average size 736 bp for sample phiM10.2 and 1096 bp for sample phiM10.1 were isolated by electrophoresis on Bio-Rad Low Range Ultra Agarose and amplified for 8 to 10 cycles with NEB High-Fidelity 2X master mix and NEBNext multiplex oligonucleotides. Library size distribution was measured on an Agilent Bioanalyzer high-sensitivity chip, and quantity was determined with the KAPA Biosystems Library Quantification kit. Paired-end 300-base sequence reads were generated on an Illumina MiSeq with a 600-cycle MiSeq v3 Reagent kit. Genome assembly from MiSeq reads was performed with Lasergene SeqMan Pro v. 11.2.1.25 (DNAStar, Madison, WI). For both phiM10.2 and phiM10.1 the coverage was approximately 5000x. The assemblies predicted for phiM10.2 and phiM10.1 have only one mismatch out of 150041 bp. Both assemblies are circularly permuted.

Submitted by: Florida State University

Study: Sinorhizobium phage vB_SmelM_phiM10 isolate:10.2 Genome sequencing

PRJNA450990 • SRP159686 • All experiments • All runs

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Sinorhizobium_phage_vB_SmelM_phiM10, T4-like Myovirus infecting Sinorhizobium meliloti 1021

Sample:

SAMN08956227 • SRS3743344 • All experiments • All runs

Organism: Sinorhizobium phage vB_SmelM_phiM10

Library:

Name: phiM10.2

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 1.3M spots, 775.2M bases, 433.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR7790243	1,309,775	775.2M	433.8Mb	2020-05-23

ID:: 6274280

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