Illumina WGS of Discolaimus major (Nematoda) - SRA

SRX18725102: Illumina WGS of Discolaimus major (Nematoda)
1 ILLUMINA (Illumina NovaSeq 6000) run: 45.5M spots, 13.6G bases, 3.9Gb downloads

Design: DNA was extracted in-house from multiple individuals (cultures) for nematode and tardigrade species, or from single specimens for all other taxa. For microfauna and mesofauna, the whole specimen was used for DNA extraction, while body parts of macrofauna were used. A non-destructive DNA extraction protocol was followed, according to Gilbert et al. (2007), except for Illumina batch 8 where the standard DNeasy Tissue Kit was used (Qiagen Inc.). Library preparations were performed in-house for earlier Illumina batches, and directly at Novogene from batch 7 onwards according to the NEBNext Ultra II DNA Library Prep Kit. Gilbert MTP, Moore W, Melchior L, Worobey M (2007) DNA Extraction from Dry Museum Beetles without Conferring External Morphological Damage. PLoS ONE 2(3): e272. doi:10.1371/journal.pone.0000437

Submitted by: LOEWE Centre for Translational Biodiversity Genomics

Study: Soil invertebrates from the LOEWE TBG and Senckenberg project 'Metagenomic monitoring of soil communities' (MetaInvert)

PRJNA758215 • SRP334402 • All experiments • All runs

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The MetaInvert database serves to improve species identification through shotgun metagenomics, to reveal evolutionary relationships, special adaptations, and host-microbiome associations. We are developing and adapting molecular approaches to study the structure, composition and functions of hard-to-observe organisms.Sequences were generated within Senckenberg and the LOEWE Centre for Translational Biodiversity Genomics (TBG) with funding from LOEWE in the MetaInvert project, and as part of the Central-European Soil Invertebrate Genome Initiative (SIGI). The vouchers are deposited in the collections of the Senckenberg Museum of Natural History Goerlitz (SMNG), Germany. The species were identified by the taxon experts of SMNG and ECT Oekotoxikologie GmbH, Florsheim, Germany.

Sample:

SAMN25040776 • SRS16166256 • All experiments • All runs

Organism: Discolaimus major

Library:

Name: b4__MI_396

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 45.5M spots, 13.6G bases, 3.9Gb

Run	# of Spots	# of Bases	Size	Published
SRR22764633	45,498,524	13.6G	3.9Gb	2023-11-16

ID:: 25804698

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