UCE target enrichment of Cerianthus membranaceus_HMC_SAMN07774929 - SRA

SRX3289592: UCE target enrichment of Cerianthus membranaceus_HMC_SAMN07774929
1 ILLUMINA (Illumina HiSeq 3000) run: 4.2M spots, 1.3G bases, 600.6Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit with dual indexed iTru adapters (Glenn et al. 2016). The resulting DNA libraries were combined in equimolar pools of 5 to 8 samples. Pooled libraries were enriched for UCE loci using the anthozoa-v1 bait set (Quattrini et al. 2017) synthesized by MYcroarray (Ann Arbor, MI). We quantified each enriched pool, post- PCR amplification, using a Qubit fluorometer and checked them for adapter contamination using a Bioanalyzer. If adapters were found, a 1X bead cleanup was performed. After cleaning up any remaining adapter contamination, we qPCR quantified each pooled library using the Kapa qPCR quantification kit, and we combined pooled libraries prior to sequencing.

Submitted by: Harvey Mudd College

Study: 33 species of Anthozoa Raw sequence reads

PRJNA413622 • SRP120054 • All experiments • All runs

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100 bp PE Illumina data for 33 anthozoans

Sample: Invertebrate

SAMN07774929 • SRS2598943 • All experiments • All runs

Organism: Cerianthus membranaceus

Library:

Name: Cerianthus membranaceus_HMC_SAMN07774929

Instrument: Illumina HiSeq 3000

Strategy: WGS

Source: GENOMIC

Selection: Hybrid Selection

Layout: PAIRED

Runs: 1 run, 4.2M spots, 1.3G bases, 600.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR6178980	4,155,997	1.3G	600.6Mb	2017-12-01

ID:: 4613495

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