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SRX3199997: UCE target enrichment of Scapanus_townsendii_LSUMZ_31414: muscle/liver tissue
1 ILLUMINA (Illumina HiSeq 3000) run: 7.3M spots, 2.2G bases, 908.4Mb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit for Illumina platforms with dual indexed iTru adapters (Glenn et al. 2016). We combined the resulting DNA libraries in equimolar pools of eight samples and enriched each pool for 5,060 UCE loci using the Tetrapods-UCE-5Kv1 probeset(Faircloth etal. 2012) sold byMYcroarray (Ann Arbor, MI). We determined the size distribution of enriched libraries with an Agilent Bioanalyzer and removed remaining adapter dimer from pools where it was present using a 0.8 bead cleanup. We then quantiÞed the enriched libraries with a Qubit 2.0 ßuorometer and pooled them in equimolar ratios.
Submitted by: Louisiana State University
Study: Phylogenetic relationships of mammals inferred using ultra conserved elements
PRJNA390442 • SRP118182 • All experiments • All runs
show Abstracthide Abstract
Our goal is to improve resolution of the phylogenetic relationships among mammals.
Sample:
SAMN07542997 • SRS2527000 • All experiments • All runs
Organism: Scapanus townsendii
Library:
Name: Scapanus_townsendii_LSUMZ_31414
Instrument: Illumina HiSeq 3000
Strategy: WGS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 7.3M spots, 2.2G bases, 908.4Mb
Run# of Spots# of BasesSizePublished
SRR60530617,297,3552.2G908.4Mb2017-09-20

ID:
4503741

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