RNA-Seq of Leptochelia sp. - SRA

SRX2458824: RNA-Seq of Leptochelia sp.
1 ILLUMINA (Illumina HiSeq 2500) run: 24.2M spots, 7.3G bases, 3.8Gb downloads

Design: Total RNA was extracted using TRIzol. mRNA was purified with magnetic oligo(dt) coated Dynabeads. Fragmented cDNA library with 200 bp fragment sizes was constructed using SuperScript III reverse transcriptase and the PrepX library preparation kit on an Apollo 324. The PrepX kit was used for indexing with indices: AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTG, GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT (indices are reverse-complimented to be used directly in Trimgalore). The library was PCR amplified with the KAPA Library Amplification kit and assessed and quantified with the HSDNA Bioanalyzer and qPCR (KAPA Library Quantification). The speciemen has been registered with the Museum of Comparative Zoology, and can be accessed at http://mczbase.mcz.harvard.edu/guid/MCZ:IZ:74245

Submitted by: Harvard University

Study: Phylogeny of Tetraconata with special focus on Crustacea

PRJNA358279 • SRP095961 • All experiments • All runs

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The goal of this study is to use large-scale transcriptome analyses to elucidate the phylogenetic relationships within Tetraconata with an emphasis on “Crustacea” and Malacostraca and Branchiopoda therein.

Sample:

SAMN06174162 • SRS1891778 • All experiments • All runs

Organism: Leptochelia sp. MCZ IZ 74245

Library:

Name: MCZ IZ 74245

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 24.2M spots, 7.3G bases, 3.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR5140116	24,170,562	7.3G	3.8Gb	2018-02-01

ID:: 3571186

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