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SRX1277820: Transcriptome of Ozophora sp. KJ-2015
1 ILLUMINA (Illumina HiSeq 2500) run: 26.4M spots, 5.3G bases, 2.3Gb downloads

Design: "Construction of libraries and sequencing on the Illumina HiSeq2500 were performed at the W. M. Keck Center for Comparative and Functional Genomics at the University of Illinois at Urbana-Champaign. Messenger RNA was selected from one microgram of high quality total RNA. RNAseq libraries were constructed using the TruSeq Stranded RNA Sample Preparation Kit (Illumina, San Diego, CA). Libraries were sequenced on an Illumina HiSeq2500 with TruSeq SBS sequencing kits version 4. The libraries were sequenced from both ends of the molecules to a total read length of 100nt from each end. The raw .bcl files were converted into demultiplexed fastq files with Casava 1.8.2 (Illumina)."
Submitted by: University of Illinois at Urbana-Champaign
Study: Hemipteroid Insect Assembling the Tree of Life Project
show Abstracthide Abstract
The hemipteroid insect orders (Hemiptera, Psocodea, and Thysanoptera) are a diverse group among the insects, including over 120,000 described species. This group contains many species of ecological, economic, or medical importance, and many of the lineages within this group have piercing-sucking mouthparts. The backbone phylogenetic relationships of this group have not been well studied. One of the major goals of this project is to combine transcriptome and genome sequences (using Illumina sequencing technology) for representatives of all the major lineages of this group and most families to construct a well resolved phylogenetic tree.
Sample:
SAMN04101422 • SRS1082287 • All experiments • All runs
Library:
Name: RhspBR5
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Spot descriptor:
forward101  reverse

Experiment attributes:
Assembly software and version: SOAPdenovo-Trans v1.02
Runs: 1 run, 26.4M spots, 5.3G bases, 2.3Gb
Run# of Spots# of BasesSizePublished
SRR249666126,436,4635.3G2.3Gb2020-02-27

ID:
1827337

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