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SRX7845184: RNA-seq of Oxymirus cursor: larval midgut
1 ILLUMINA (Illumina HiSeq 2500) run: 84M spots, 25.4G bases, 9.4Gb downloads

Design: Larvae were sterilized by 70% ethanol and rinsed in double distillated water. Larvae were opened ventrally to expose the digestive tract and midgut were dissected out. Dissected gut were extensively washed in sterile water to remove gut content and frozen in liquid nitrogen and stored at -80C until further use. Total RNA was extracted from the dissected midguts using Trizol and DNA contamination was removed by DNAse treatment. Total RNA samples were purified by using RNeasy MinElute Clean up Kit and quantified using the RNA 6000 Nano LabChip kit on an Agilent 2100 Bioanalyzer. 150bp paired-end sequencing was performed on an Illumina Hiseq 2500 platform.
Submitted by: Max Planck Institute for Chemical Ecology
Study: Investigating the distribution of digestive enzymes in xylophagous larvae of the family Cerambycidae
show Abstracthide Abstract
Larvae of longhorned beetles (Coleoptera; Cerambycidae) live in challenging environments, as they feed on woody tissues made up of large amounts of plant cell wall. Cerambycid larvae have to break down plant cell wall polysaccharides to efficiently access nutrients, which require them to possess plant cell wall degrading enzymes (PCWDEs) in their digestive tracts. To date, molecular data for Cerambycidae are only available for the subfamily Lamiinae, and our understanding of the distribution and the evolutionary history of PCWDEs in Cerambycid are limited for this beetle family. To extend the knowledge on distribution and evolution of PCWDEs in Cerambycidae, we collected larval specimens mostly in Europe representing six out of eight recognized subfamilies in the family Cerambycidae. We performed RNA-Seq using RNA isolated from larval guts. We then analyzed the complement of PCWDEs for each species.
Sample:
SAMN14253357 • SRS6250927 • All experiments • All runs
Organism: Oxymirus cursor
Library:
Name: OCU
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: PolyA
Layout: PAIRED
Runs: 1 run, 84M spots, 25.4G bases, 9.4Gb
Run# of Spots# of BasesSizePublished
SRR1123335283,951,99625.4G9.4Gb2021-06-14

ID:
10263212

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