show Abstracthide AbstractLarvae of longhorned beetles (Coleoptera; Cerambycidae) live in challenging environments, as they feed on woody tissues made up of large amounts of plant cell wall. Cerambycid larvae have to break down plant cell wall polysaccharides to efficiently access nutrients, which require them to possess plant cell wall degrading enzymes (PCWDEs) in their digestive tracts. To date, molecular data for Cerambycidae are only available for the subfamily Lamiinae, and our understanding of the distribution and the evolutionary history of PCWDEs in Cerambycid are limited for this beetle family. To extend the knowledge on distribution and evolution of PCWDEs in Cerambycidae, we collected larval specimens mostly in Europe representing six out of eight recognized subfamilies in the family Cerambycidae. We performed RNA-Seq using RNA isolated from larval guts. We then analyzed the complement of PCWDEs for each species.