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SRX7138425: target enrichment of UCEs and exons from anthozoans
1 ILLUMINA (Illumina NovaSeq 6000) run: 11.6M spots, 3.5G bases, 1.2Gb downloads

Design: We prepared DNA libraries using the Kapa Biosystems Hyper Prep Kit with dual indexed iTru adapters (Glenn et al. 2016). The resulting DNA libraries were combined in equimolar pools of 5 to 8 samples. Pooled libraries were enriched for UCE loci using the anthozoa-v1 bait set (Quattrini et al. 2017) synthesized by MYcroarray (Ann Arbor, MI). We quantified each enriched pool, post- PCR amplification, using a Qubit fluorometer and checked them for adapter contamination using a Bioanalyzer. If adapters were found, a 1X bead cleanup was performed. After cleaning up any remaining adapter contamination, we qPCR quantified each pooled library using the Kapa qPCR quantification kit, and we combined pooled libraries prior to sequencing.
Submitted by: Harvey Mudd College
Study: Phylogenomics of Anthozoa: New Approaches to long-standing problems
show Abstracthide Abstract
Sequence data were captured using a targeted enrichment approach for UCEs and exons.
Sample:
SAMN13244987 • SRS5647094 • All experiments • All runs
Library:
Name: ANT289B
Instrument: Illumina NovaSeq 6000
Strategy: WGS
Source: GENOMIC
Selection: Hybrid Selection
Layout: PAIRED
Runs: 1 run, 11.6M spots, 3.5G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
SRR1044380111,607,9343.5G1.2Gb2020-05-01

ID:
9383368

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